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张海英, 杨莉, 梁艺怀, 黄明立, 金泰廙. HSP 70各亚型在染尘体外细胞模型中表达[J]. 中国公共卫生, 2009, 25(8): 979-981. DOI: 10.11847/zgggws2009-25-08-46
引用本文: 张海英, 杨莉, 梁艺怀, 黄明立, 金泰廙. HSP 70各亚型在染尘体外细胞模型中表达[J]. 中国公共卫生, 2009, 25(8): 979-981. DOI: 10.11847/zgggws2009-25-08-46
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ZHANG Hai-ying, YANG Li, LIANG Yi-huai, . HSP70 subtypes expression in human embryonic lung fibroblasts induced by supernatants of alveolar macrophage exposed to silica[J]. Chinese Journal of Public Health, 2009, 25(8): 979-981. DOI: 10.11847/zgggws2009-25-08-46
Citation: ZHANG Hai-ying, YANG Li, LIANG Yi-huai, . HSP70 subtypes expression in human embryonic lung fibroblasts induced by supernatants of alveolar macrophage exposed to silica[J]. Chinese Journal of Public Health, 2009, 25(8): 979-981. DOI: 10.11847/zgggws2009-25-08-46
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HSP 70各亚型在染尘体外细胞模型中表达

HSP70 subtypes expression in human embryonic lung fibroblasts induced by supernatants of alveolar macrophage exposed to silica

    摘要
  • 摘要: 目的 观察染尘巨噬细胞上清液对人胚肺成纤维细胞热休克蛋白70(HSP70)各亚型(HSP70-1、HSP70-2、HSP70-hom)表达的影响。方法 采用大鼠巨噬细胞(AMs)和人胚肺成纤维细胞(HELF)构成体外模型,采用逆转录多聚酶链反应(RT-PCR)技术检测二氧化硅粉尘处理AMs24h的培养上清液刺激HELF细胞的HSP70各亚型表达情况。同时用二氧化钛作为阴性对照,以不用粉尘处理的AM培养上清液作为正常对照。结果 HSP70-1、HSP70-2、HSP70-hom基因表达与粉尘浓度的相关系数(R)依次为0.931(P<0.01)、0.923(P<0.01)、0.818(P<0.05),呈现明显的剂量-反应关系。结论 受染尘肺泡巨噬细胞上清液刺激的人胚肺成纤维细胞HSP70各亚型在转录水平呈表达增加,且可能参与了矽尘致细胞氧化应激过程。

     

    Abstract: Objective To observe the expression of HSP70-1,H SP70-2,HSP70-hom in human embryonic lung fibroblasts(HELF)induced by supernatants of alveolarm acrophage(AMs)exposed to silica.Methods AMs and HELF of rats were formed invitro model.The HSP70-1,HSP70-2,HSP70-hom mRNA levels of H ELF stmiulated by supernatants of AMs exposed to silica were detected by RT-PCR technology.Results Expression of HSP70-1,HSP70-2,HSP70-hom could be induced by dust-exposed AMs and there was a clear dose-effectrelationship between HSP70-1,HSP70-2,HSP70-hom mRNA levels and silica concentration with the correlation coefficients of 0 .931(P<0. 01),0. 923(P<0 .01),0. 818 (P<0.05),respectively.Conclusion HSP70-1,HSP70-2,HSP70-hom may play an antioxidant role in the process of oxidative stress induced by silica.

     

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