Abstract: Objective To survey the antibiotic resistance and the prevalence for penicillina seproducing Neisseria gonorrhoeae(PPNG)and potential tetracy cline resistance of Neisseria gonorrhoeae(TRNG)in Shenzhen.Methods Min miuminhibitory concentration(MIC)to 240 strains Neisseria gonorrhoeae of penicillin,tetracyc line,cipro floxacin,speeotinomy cinand ceferiaxone were determined with agard ilution method.PPNG strain swere determined with m dified quantitative iodine method.Results The percentage Neisseria gonor rhoeae strains resisteant to penicillin,tetracy cline,cipro floxac in and ceferi axone was 7.67%,78.33%,37.50%,and 667%,respectively.There were 32(13.33%)PPNG strains and 30(12.5%)TRNGstrains.31.67% of theiso lates were smiultaneously resistant to penic llin,tetracy cline and cipro floxacin.Conclusion Ceferiaxone and speeotinomy cin could be given the priority to treat patients with Neisseria gonor rhoeae.There sistant strains of Neisseria gonorrhoeae to penicillin and tetracyc line are prevalent.