Abstract: Objective To investigate an timicrobial resistance of Enterococcus isolated in the First Clinical Hospital of Jilin University during 2003-2007.Methods Disk diffusion testwas used to study the antmiicrobial resistance.The results were evaluated based on Clinical and Laboratory Standards In stitute.WHONET 5 was applied for analysis.Results Most of 642 Enterococcus strains were collected from urine(44.4%),respiratory secretion specimen(21.8%),secretions and abscesses(18.7%)and blood(8.6%)during 5 years.The rates of high level am no glycosides resistant(HLAR)in Enterococcus faecalis and Enterococcus faecium was 57.4%-72.5% and 72.1%-93.8%,respectively.There was novancomy cin and teicoplanin resistant strain.Susceptibility test showed thatEntero coccus wasmultiple resistant.During the 5 years,the antmi icrobial resistance of Entero coccus increased.Conclusion Monitoring of the antimicrobial resistance of Enterococcus sho ld be streng thened.The change of the antmi icrobial resistance should be investigated in order to direct rational drugus age inclinic.