巨噬细胞损伤型大鼠矽肺纤维化模型

Silicosis fibrosis model through macrophage injury in rats

摘要: 目的研究巨噬细胞接触SiO₂颗粒后,引起巨噬细胞破坏、崩溃的可能性及程度。方法将Wistar大鼠一次性腹腔注入1ml灭菌的SiO₂颗粒溶液,第1~3 d分别抽取腹腔液,显微镜下观察巨噬细胞吞噬SiO₂颗粒以及巨噬细胞破坏的情况。结果用SiO₂颗粒腹腔注入4 h后可以造成巨噬细胞典型的细胞病变、破坏、最后崩溃,并且巨噬细胞的破坏率可达10%以上。结论应用本模型可以研究矽肺纤维化时巨噬细胞在吞噬SiO₂颗粒时的病变、信号、信号传导、基因改变、蛋白质变化等。为矽肺致病机制的研究提供了一种简便,客观,容易操作的矽肺模型。

Abstract: ObjectiveTo study the possibiliy and degree of macrophage injury and collapse induced by SiO₂.Methods Sterile SiO₂ solution(1ml)was injected in to abdominal cavity of Wistar rats one time.The peritoneal fluids of the rats was taken at 1st-3rd day after the exposure to observe the phayocytose and injuries of macrophages.ResultsTypical cytopathic,damage and finally breakdown resulted from the injection of SiO₂ particles were observed 4 hours after the injection.The damage rate of macrophage was up to 10%.ConclusionThe animal model of silicos is was established and could be adopted in pathogenic study of silicosis.