高级检索
戴佳琳, 黄江, 廖兴江, 周灵贵, 申萍香, 刘玉江, 郎书源. 猪带绦虫腺苷酸激酶基因及蛋白结构特性分析[J]. 中国公共卫生, 2009, 25(10): 1214-1215. DOI: 10.11847/zgggws2009-25-10-33
引用本文: 戴佳琳, 黄江, 廖兴江, 周灵贵, 申萍香, 刘玉江, 郎书源. 猪带绦虫腺苷酸激酶基因及蛋白结构特性分析[J]. 中国公共卫生, 2009, 25(10): 1214-1215. DOI: 10.11847/zgggws2009-25-10-33
DAI Jia-lin, HUANG Jiang, LIAO Xin-jiang, . Prediction and analysis for structure and function of adenylate kinase gene from Taenia solium by bioinformatics[J]. Chinese Journal of Public Health, 2009, 25(10): 1214-1215. DOI: 10.11847/zgggws2009-25-10-33
Citation: DAI Jia-lin, HUANG Jiang, LIAO Xin-jiang, . Prediction and analysis for structure and function of adenylate kinase gene from Taenia solium by bioinformatics[J]. Chinese Journal of Public Health, 2009, 25(10): 1214-1215. DOI: 10.11847/zgggws2009-25-10-33

猪带绦虫腺苷酸激酶基因及蛋白结构特性分析

Prediction and analysis for structure and function of adenylate kinase gene from Taenia solium by bioinformatics

  • 摘要: 目的分析和预测猪带绦虫腺苷酸激酶(ADK)基因及其编码的蛋白结构和特性。方法利用各种在线生物信息网站及其它分析软件包,分析从猪带绦虫成虫cDNA质粒文库中识别的腺苷酸激酶基因的结构和编码区序列,分析、预测编码的蛋白质的理化特性、抗原表位、翻译后的修饰、功能域、亚细胞定位、拓扑结构、二级结构、三维空间构象等。结果该基因全长864 bp,编码区为110~758,编码215个氨基酸,为全长基因。理论分子量、等电点分别为23771.4 Da和6.86;没有跨膜区;三维结构建模图显示其线性表位的部位。结论应用生物信息方法从猪带绦虫成虫cDNA文库中筛选出了腺苷酸激酶基因的cDNA全长序列并预测得到其结构与功能方面的信息。

     

    Abstract: ObjectiveTo analyze and predict the structure and characteristics of adenylate kinase gene from Taenia solium by bioinformatics for its biological function and application research.MethodsWith analyzing tools of bioinformatics in webs site and bioinformatics software package,adenylate kinase was recognized from the Taenia solium full-length cDNA plasmid libratory andits gene structure and coding region sequence was analyzed andits physical-chemical characteristics,topo logical structure and 3 Dstructure were predicted.ResultsThe full gene is 864 bp in length and its coding region is 110-758,coding 215 amino acids.It is a complete full-length gene comparing with the homologues in Gen Bank.The prote in has no transmem brane region.The molecular weight of adeny late kinase gene is predicted to be 23771.4Da.The proe in contains no transmem brane region and subcellular location sequecce.The predicted amino acid sequence shows 66% identity and 84% smiilarity with that of ADK form Schisto somaja ponicum.ConclusionThe cDNA sequence encoded adenylate kinase gene was screened from cDNA library of adult Taenia solium by bioinformatical method.The gene and prote in structure and characteristics of Taenia soliumadeny late kinase were obtained.

     

/

返回文章
返回