抗坏血酸对染砷小鼠肝脏氧化损伤拮抗作用
Antagonistic effect of ascorbic acid on oxidative damage induced by arsenic in mice
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摘要: 目的 研究抗坏血酸(VC)对砷中毒小鼠肝脏中丙二醛(MDA)、超氧化物歧化酶(SOD)和谷胱甘肽(GSH)的影响。方法 用硫代巴比妥酸法测定丙二醛(MDA)含量;用Nitrite-kit法检测SOD活性;用二硫双硝基苯酸(DTNB)比色法测定GSH含量。结果 对照组MDA含量为14.87 nmol/(mg·prot),SOD活力为167.49NU/(mg·prot),GSH含量为51.27 nmol/(mg·prot);20μmol/L染砷组小鼠肝脏中MDS含量明显增高,为18.48nmol/(mg·prot),SOD活力明显下降,为125.97 NU/(mg·prot),GSH含量也显著降低,为34.87 nmol/(mg·prot);而抗坏血酸拮抗组小鼠肝脏中MDS含量与单纯染砷组相比明显下降,为16.24 nmol/(mg·prot),但未达到对照组水平;SOD活力明显增高,为138.94 NU/(mg·prot),但未达到对照组水平,GSH含量显著增高,为50.39 nmol/(mg·prot),与对照组比较差异无统计学意义。结论 抗坏血酸可拮抗砷对小鼠肝脏的氧化损伤。Abstract: Objective To explore the effects of ascorbic acid on the content of MDA，activity of SOD and content of CSH in arsenic poisoning mice.Methods The malonaldehyde (MDA)content was detected by thio-barbituric acid method Superoxide dismutase(SOD)activity was deteun fined by nitrite-kit Clutathione (CSH)content was detected by dinitrothiocyanobenzene chromatometry.Results The content of MDA in control group was 14.87 nmol/mg·prot SOD activity was 167.49 NU/mg·prot CSH contentwas 51.27 nmo1/mg·prot In 20μmol/L,arsenite treated group,the MDA increased to 18.48 nmol/mg·prot SOD activity decreased to 125.97NU/mg·prot and CSH content decreased to 34.87 nmol/mg·prot.In the ascorbic acid treated group,the MDA content decreased to 16.24nmol/mg·prot SOD activity increased to 138.94 NU/mg·prot CSH content increased to 50.39 nmol/mg·prot.Conclusion Ascorbic acid can alleviate the oxidative damage induced by arsenic in mice liver.
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