阴道毛滴虫ITS序列多态性与甲硝唑耐药性
Association between rDNA-ITS polymorpholism and metronidazole resistance in Trichomonas vaginalis
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摘要: 目的 探讨临床不同分离株阴道毛滴虫核糖体DNA基因转录间隔区(rDNA ITS)序列多态性与甲硝唑抗性表型之间的相关性。方法 采用微孔板法检测39株达到纯培养的阴道毛滴虫虫株对甲硝唑敏感性,同时设计rDNA-ITS区特异引物进行PCR扩增并测序。结果 39个虫株中,甲硝唑敏感虫株20株,耐药虫株19株;PCR扩增获得ITS区和5.8 S特异片段长度为285 bp。经DNAStarMeg Align比对显示,仅在ITS1区存在唯一点突变,即C66T突变。39个虫株中有8株具C66T突变,其中6株来自甲硝唑敏感虫株,2株来自甲硝唑耐药虫株。经Fisher确切概率检验,χ2=0.108,P>0.05。结论 阴道毛滴虫临床分离株ITS区C66T突变与甲硝唑抗性表型无明显相关性。Abstract: Objective To study there lationship between the polymorpholism of rDNA-ITS and the resistance to metronidazole in Trichomonas vaginalis.Methods Thirty-nine clinical isolated strains of Trichomonas vaginalis were detected for resistance to metronidazole in aerobic condition with 96-wells plates and rDNA-ITS were amplified and sequenced.Results Twenty strains were metronidazole susceptible and 19 strains were metronidazole resistant.A285bp rDNA-ITS and 5.8S fragment were amplified and sequenced.Only one point mutation(C66T)was detected by sequence a lignment with MegA lign of DNA Star softw are.A total of 8 stra ins were detected in which 6 strains were from metronidazole-susceptible group and the other 2 from the metronidazole resistant group.There was no statistics difference between susceptible and resistant strains with Fishe rexact test(P>0.05,χ2=0.108).Conclusion There is no obvious association between C66T mutation of rDNA-ITS and resistance to metronidazole in T.vaginalis.
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