慢性染铝对大鼠海马长时程增强及Ca2+浓度影响

周平; 唐秋实; 靳翠红; 姜杰; 时利德; 蔡原

doi:10.11847/zgggws2009-25-12-46

慢性染铝对大鼠海马长时程增强及Ca2+浓度影响

Effect of postweaning chronic aluminum exposure on long-term potentiation and intracellular Ca2+ concentration in hippocampus of rats

摘要

摘要: 目的探讨慢性铝暴露对断乳后大鼠海马CA1区长时程增强及细胞内Ca²⁺浓度的影响.方法 54只大鼠随机分为3组,对照组(蒸馏水)和实验组(0.2%,0.6%AlCl3溶液).采用自由饮水方式染毒,连续80~90 d.测定血铝和脑铝含量、大鼠海马CA1区群体锋电位和大鼠海马细胞内Ca²⁺浓度.结果各铝暴露组血铝和脑铝均明显高于对照组(P<0.01);高频刺激后,0.2%AlCl3组海马CA1区群体锋电位幅值增强率为117.70%;0.6%AlCl3组为109.77%,均明显低于对照组(P<0.01);且有随铝暴露浓度增加,群体锋电位幅值增强率逐渐降低的趋势;0.2%与0.6%AlCl3组海马细胞内Ca²⁺浓度分别为(228.38±51.74)和(195.43±36.95)nmol/L,与对照组比较,差异有统计学意义(P<0.01).结论铝所致海马细胞内Ca²⁺浓度的降低可能是其影响长时程增强的机制之一.

Abstract: Objective To explore the effect of aluminium on longterm potentiation(LTP)in hippo campal CA1 area and intracellular Ca²⁺ concentration in postweaning rats.Methods Postweaning Wistar rats were administered with aluminium chlor ide in water at the doses of 0.2% and 0.6% for 80-90 days.A tomic absorption spectrometry(AAS)was used to detect the content of a luminium in blood and brain.The technique of extrace llula relec trophy siolo gicaltest was used to record LTP in hippocampal CA1 area and Fura 2/AM calciumions fluo rescence indicator was used to measure in trace llular Ca²⁺ concen tration in hippocampal cells.Results The contents of alum inium in blood and brain of a luminium exposed rats were rem arkably higher than that of the control group.The population spike(PS)of LTP in hippo campalCA1 area and the intrace llular Ca²⁺ concentration were significantly reduced in aluminium exposed groups.Conclusion Aluminium can impair the induction and maintenance of LTP in hippo campal CA1 area,and the impairmentm aybe results from the reduction of intrace llular Ca²⁺ concen tration.

HTML全文

参考文献(0)

施引文献

资源附件(0)