Abstract: Objective To investigate sub-health status and its related factors among medical freshmen for founulating preventive measures of sub-health.Methods Sub-health status was assessed by a Self-rated Health Measurement Scale among 2554 freshm en in Guangdong Medical University.The diagnosis of sub-health included physical sub-health,psychological sub-health and inadaptability to the society.We calculated prevalence rate of sub-health and deteun fined factors related to sub-health using chi-square test and multiple logistic regression model.Results The prevalence mte of sub-health was 40.20%.The rate of physical sub-health was the highest,followed by physical inadaptability and psychological inadaptability Am ong the students from other provinces,fem ales had a higher prevalence mte of sub-health(χ²=18.482,P=0.030).Among the students from Guangdong,the difference between gender was not statistically significant(χ²=16.932,P=0.076).Physical fatigue,mental tiredness,cold,eyes fatigue,dry eyes,insomnia,anorexia,and dyspepsia were hazards to new students' health Negative emotion,depression,anxiety,and pain had negative inpact on psychological health; low efficiency in study was also an inportant risk factor affecting freshmen's psychological health.Conclusion Sub-health among freshmen of medical university exists widely Universities need to pay attention to prevent the occurrence of freshmen's subhealth.