氟对大鼠肝功及GSH-Px基因表达影响
Effect of fluoride on liver function, expression of GSH-Px gene and ultrastructure of liver cell in rats
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摘要: 目的 探讨不同浓度氟对大鼠肝功和谷胱苷肽过氧化物酶(GSH-Px)基因表达及肝细胞超微结构影响。方法 在饮水中加入不同剂量氟化钠喂饲大鼠30 d后,测定血清氟含量、门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)活力、肝组织中GSH-Px活性及mRNA表达,观察大鼠肝细胞超微结构。结果 随染氟剂量增加,各剂量组大鼠血氟浓度逐渐增加,血清中AST、ALT含量逐渐增加,呈剂量-效应关系(R=0.889,R=0.729,P<0.01);GSH-Px酶活力单位从(19.672±1.025)下降至(11.777±1.529),与对照组比较,差异有统计学意义(P<0.01);GSH-Px的mRNA表达下降(P<0.05)。中、高剂量组大鼠肝细胞超微结构可见线粒体肿胀,有较多脂肪变性。结论 过量氟可能诱导GSH-Px的mRNA表达下降,使大鼠肝脏抗氧化系统受到抑制。Abstract: Objective To study the effects of different dosages of fluoride on liver function,expression of GSH-Px gene,and the ultrastruc ture of liver cell in rats.Methods Drinking water with various concentrations of sodium fluoride was given to rats for 1 month.The contents of the fluoride,the activities of asparate aminotransfe rase(AST),alamine aminotransferase(ALT),glutathion peroxidase(GSH-Px),and mRNA of GSH-Px in liver of the rats were measured.Results The serum fluoride increased in dose 2effect manner with the fluoride exposure.The activities of AST and AL T of experimental groups increased with a dose-effect trend compared with that of the control group.The mRNA of GSH-Px of liver was decreased with the fluoride exposure.The swollen of mitochondrion and degeration of fatty cells were observed in middle and high dose fluoride exposure groups.Conclusion Fluoride exposure may induce decreased expression of mRNA of GSH-Px and restrained antioxidation function of liver in rats.
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