Abstract: Objective To investigate common antibiotics resistance of Helicobacter pylori(Hp) isolated from patients in Lanzhou city and to provide the clinical guidelines for choosing effective agents for Hp eradication therapy.Methods Gastric mucosa tissues of the patients with upper gastroenterol diseases were sampled to isolate and identify Hp.Kirby-Bauer test was performed to test the resistance of isolated Hp strains to amoxicillin,clarithromycin,metronidazole,furaltadon,and levofloxacin.Results Totally 81 strains of Hp were isolated from 197 samples(41.12%) and there was no difference in the isolation rate between the patients with different sex and age(P>0.05).The positive rate of Hp infection in patients with peptic ulcer was significantly higher than that of patients with chronic gastritis(P<0.05).The resistant rate of Hp to amoxicillin,clarithromycin,metronidazole,furaltadon,and levofloxacin was 3.70%,7.41%,67.90%,3.70%,and 6.17%,respectively,and with no difference between isolations form patients with different sex,age and the pathological type.Conclusion Metronidazole should not be used as first-line agent for Hp treatment in Lanzhou area due to its higher resistance rate.Furaltadone and amoxicillin should be used first for Hp treatment.