HBsAg携带者C型乙型肝炎病毒PreS基因序列建立

Establishment of consensus sequence of PreS of hepatitis B virus genotype C prevailing in Nantong city

摘要: 目的建立南通地区C型乙型肝炎病毒(HBV)流行株PreS基因参考序列。方法扩增25例HBV慢性无症状携带者的PreS基因,分析并确定基因型,选同HBVC亚型的PreS拟定参照序列。结果将所测基因序列与HBV不同型PreS序列用Clustalx1.8软件对比,经MEGA3.1软件分型。结果表明,其中有19份血样属C型,有6份血样属B型;南通地区C型HBV流行株的PreS参照序列与GenBank收录的HBV-C型序列D50517.1以及重庆地区的参照序列进行比较,分别有6个核苷酸和10个核苷酸位点的差异,同源性分别为98.9%和98.1%。结论建立了南通地区C型HBV流行株的PreS基因参照序列。

Abstract: ObjectiveTo develop consensus sequence of C subtype of HBV PreS in Nantong city.MethodsThe gene of HBV PreS was amplified with PCR and sequenced.The main strain prevailing of HBV subtype C was identified to establish its consensus sequence.The sequences of all samples were analyzed using Clustalx1.8 and MEGA3.1.ResultsThe results showed that 19 strains were genotype C and 6 were genotype B.The consensus of PreS of HBV subtype C was established.There were 6 and 10 different sites of base page comparing HBV-C D50517.1 and Chongqing consensus sequence.The homologies of the gene were 98.9% and 98.1%,respectively.ConclusionThe consensus sequence of PreS of HBV subtype C of Nantong city was established.