矽尘对人胚肺成纤维细胞MMP9/TIMP1表达影响

MMP9 and TIMP1 expression in human embryonic lung fibroblasts induced by supernatant of alveolar macrophage exposed to silica

摘要: 目的观察染尘细胞模型中人胚肺成纤维细胞(MRC-5)基质金属蛋白酶9(MMP9)及其组织抑制因子1(TIMP1)诱导表达的时间-效应关系。方法大鼠巨噬细胞(AMs)和MRC-5组成体外模型,采用逆转录多聚酶链反应(RT-PCR)技术检测体外染尘细胞模型中MRC-5分别在0,12,24,36,48,60 h等时段MMP9和TIMP1表达情况。结果 MMP9和TIMP1基因表达呈现明显时间-效应关系,SiO₂染尘组相关系数r分别为0.639和0.976,2基因表达存在一定时差,早期以MMP9增高为主。结论基质金属蛋白酶生物合成的变化可能发生在转录水平。

Abstract: Objective To observe the expression of metalloproteinase-9(MMP9)and tissue inhibitor of metallproteinnase-I(TIMP1)in human embryonic lung fibroblasts(MRC-5)induced by supernatant of alveolar macrophage(AMs)exposed to silica.Methods Rat AMs and MRC-5 were made into a model in vitro.MMP9 and TMIP1 mRNA levels in the induced MRC-5 were detected with RT-PCR technology at the culture time of 0,12,24,36,48,and 60 hours.Results Expression of MMP9 and TMIP1 could be induced by silica exposed AMs and there was a good time effect relationship between MMP9 and TIMP1 mRNA level and culture time.Increasing of MMP9 expression appeared earlier than TMIP1.Conclusion The result indicates that the biosyn thesis change of metalloproteinases occurs at transcription level.