Abstract: ObjectiveThree different methods of Tricine-sodium dodceyl sulfate polycarylamide gel electrophoresis (Tricine-SDS-PAGE)were used to analyze the hemolymph of Musca domestica(housefly)larvae.MethodsThe hemolymph of Musca domestica(housefly)larvae was analyzed with the separating gel without urea or glycerol in ordinary Tricine-SDS PAGE method,with the urea separating gel method,and the separating gel with glycerol method.ResultsThe results showed that there were abundant low molecular peptides in the hemolymph of housefly larvae.The separating gel without urea or glycerol in ordinary Tricine-SDS PAGE method could not effectively separate the low molecular proteins and peptides with the molecular weight below 10.700 kDa.The result of analysis with the urea separating gel showed that it could effectively separate the hemolymph of housefly larvae,especially the low molecular peptides,but the determination of its molecular weight was not accurate.And the separating gel with glycerol achieved good results not only in separating the low molecular peptides but also in determining the molecular weight.ConclusionThe method of the separating gel with glycerol could achieve satisfactory results in the determination of the hemolymph of housefly larvae.