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徐国强, 李远慧, 李娜, 钱立全. 半胱氨酸蛋白酶-3在染砷大鼠肾近端小管表达[J]. 中国公共卫生, 2010, 26(12): 1550-1551. DOI: 10.11847/zgggws2010-26-12-40
引用本文: 徐国强, 李远慧, 李娜, 钱立全. 半胱氨酸蛋白酶-3在染砷大鼠肾近端小管表达[J]. 中国公共卫生, 2010, 26(12): 1550-1551. DOI: 10.11847/zgggws2010-26-12-40
XU Guo-qiang, LI Yuan-hui, LI Na, . Expression of caspase-3 in renal proximal convoluted tubules in rats with arsenic poisoning[J]. Chinese Journal of Public Health, 2010, 26(12): 1550-1551. DOI: 10.11847/zgggws2010-26-12-40
Citation: XU Guo-qiang, LI Yuan-hui, LI Na, . Expression of caspase-3 in renal proximal convoluted tubules in rats with arsenic poisoning[J]. Chinese Journal of Public Health, 2010, 26(12): 1550-1551. DOI: 10.11847/zgggws2010-26-12-40

半胱氨酸蛋白酶-3在染砷大鼠肾近端小管表达

Expression of caspase-3 in renal proximal convoluted tubules in rats with arsenic poisoning

  • 摘要: 目的探讨慢性染砷对大鼠肾脏近端小管上皮细胞半胱氨酸蛋白酶3(caspase-3)表达的影响。方法将30只健康成年清洁级SD大鼠随机分为高、低剂量(分别为10.0,0.4 mg/kg)染砷组和对照(蒸馏水)组,采用自由饮水方式(As2O3溶于饮水中)进行染毒,染毒容量为200 mL/kg,连续染毒6个月,测血砷、尿砷含量,取大鼠肾脏进行HE染色,免疫组化法检测染砷大鼠近端小管上皮细胞caspase-3的表达,图像分析计数。结果与对照组比较,高、低剂量染砷组大鼠血砷、尿砷明显增高(P<0.01);染砷大鼠近端小管上皮细胞空泡样变性,细胞腔面刷状缘脱落并减少,可见核固缩;与对照组(1.30±1.02)个/视野比较,高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3阳性细胞数分别为(63.94±10.83)、(16.00±8.23)个/视野,明显升高(P<0.05);高、低剂量染砷组大鼠肾近端小管上皮细胞caspase-3表达灰度值分别为(90.43±11.43)、(107.94±10.72),与对照组比较,差异均有统计学意义(P<0.05)。结论慢性砷中毒可致大鼠近端小管上皮细胞caspase-3表达。

     

    Abstract: ObjectiveTo explore the relationship bewteen digitratio and aggressive behavior in male of Han nationality.MethodsTotally 1 358 male of Han nationality aged from 16 to 35 years were surveyed with revised aggression questionnaire.Of the participants,79 were selected as the violence tendency group,and 279 as normal group.Measurements were takenfrom the middle of the basal crease to the tip of the finger with electronic vernier caliper.ResultsPhysical aggression,verbal aggression,anger and hostility,and total score were evidently higher in violence tendency group than those of in normal group(P<0.001).In violence tendency group and normal group,the left index fingers(2D)were 73.02±3.55, 71.91±3.95 mm,respectively;the left ring fingers(4D)were 75.79±3.63,74.62±4.27 mm,respectively;the right ring fingers(4D)were 75.51±3.63,74.39±4.26 mm,respectively.There were significant differences in the index of left ring finger and the right ring finger between violence tendency group and normal group(P<0.05).There was no significant difference in digitratio bewteen the two groups.In violence tendency group,The ratio of 2D:3Do n-left was significantly related to verbal aggression,hostility,and total aggression(r=0.271,0.268,0.272;P<0.05).The ratio of 2D:4D on left was significantly related to verbal aggression and hostility(r=0.244,0.223;P<0.05).The ratios of 3D:4D and 4D:5D on right were significantly related to hostility(r=0.266,-0.236;P<0.05).In normal group,the ratio of 2D:4D on left is significantly related to verbal aggression(r=0.126,P<0.05)and the ratio of 3D:5D on left was significantly related to anger(r=-0.126,P<0.05).ConclusionThere is are lation partly bewteen digitratio and aggressive behavior.

     

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