北京地区福氏志贺菌4c亚型毒力基因分析

王中强; 陈琛; 刘雯静; 柳楠; 薛文仲; 李婧; 苏文莉; 邱少富; 王勇; 黄留玉; 孙岩松; 宋宏彬

doi:10.11847/zgggws2011-27-01-21

北京地区福氏志贺菌4c亚型毒力基因分析

Detection of virulence genes of Shigella flexneri 4c isolated in Beijing

摘要

摘要: 目的了解北京地区福氏志贺菌4 c亚型生化特征和毒力基因携带情况.方法对分离自北京地区的44株福氏4 c亚型志贺菌采用Api生化板条进行鉴定,玻片凝集法进行血清分型,应用PCR技术对其侵袭性质检抗原(ipaH)、志贺肠毒素(setlA、setlB)、侵袭相关位点(sen、ial)和毒力表达调控基因(virF)6种毒力基因进行检测.结果全部福氏4 c志贺菌只发酵葡萄糖、甘露醇、蜜二糖和阿拉伯糖;血清凝集抗原结构式为(Ⅳ:7,8);ipaH、setlA、setlB、sen、ial和virF6毒力基因携带率分别为100%、97.7%、100%、77.2%、72.7%和77.2%;54.5%的菌株携带全部6种毒力基因.结论福氏4 c志贺菌毒力基因携带率高,具有较强致病性.

Abstract: Objective To investigate and analyze the biochemical characteristics and virulence genes of Shigella flexneri 4c isolated in Beijing.Methods Totally 44 S.flexneri 4c isolates from diarrhea patients in Beijing were systematically determined with biochemical reactions and serological tests.Detections of ipaH,setlA,setlB,sen,ial,and virF virulence genes were performed using PCR.Results All the S.flexneri 4c isolates could ferment glucose,mannitol,melibiose,and arabinose.Presence of ipaH,setlA,setlB,sen,ial,and virF virulence genes were identified among 100%, 97.7%, 100%, 77.2%,72.7%, and 77.2% of 44 tested isolates, respectively.Conclusion Presence of virulence genes associated with strong path ogenicity was found in a high proportion of Shisella flexneri 4c isolates.

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