Abstract: Objective To study effects of selenium on glycome tabolism key enzymes and its mechanism in hepatocytes of ratswith oxidative damage.Methods Rathepatocytes were exposed to 0.1 mM H₂O₂ and then were incubated with different do sages of selenium for 24 hours.The mRNA expression of glucokine(GK), glycog ensymthase(GS), and prote in kinase B(PKB/Akt) were detected with realtime PCR.The prote in expression of PK B/A ktwsmeasured withWestern blot.Results The mRNA expression of GK in selenium groups was 9.692-16.588×10^-6, higher than that of in H₂O₂ oxida tive model group(P < 0.05).The mRNA expression of GS and Akt in high dose selenium were 57.618 and 0.2398×10^-3, higher than those of in H₂O₂ oxidative model group(P < 0.05).The expression of prote in in selenium groups was 0.3343-0.4346×10^-3, higher than that of in H₂O₂ oxidative model group(P< 0.05).Conclusion Selenium could improve the glucometabolism key enzymes GK and GS in rathepa to cyteswith oxidative damage.The mechanism of the effect may relate to that selenium could up regulate the expression of A kt which is the key molecule in insulin signal pathway.