亚慢性钐暴露对小鼠肝脏组织结构影响
Influence of subchronic samarium exposure on liver tissue structure in mice
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摘要: 目的 观察亚慢性钐暴露对小鼠肝脏组织结构的影响。方法 80只小鼠随机分成5组,对照组饮用纯净水;实验组分别饮用5、50、500和2 000 mg/L的硝酸钐水溶液,染毒90 d后处死小鼠,迅速取肝脏,准确称重后进行光镜、电镜切片观察肝组织形态学变化。结果 与对照组比较,各染毒组小鼠体重无明显差异,2 000 mg/L硝酸钐组肝指数明显低于对照组;光镜检查500 mg/L硝酸钐组肝细胞肿大,轻度变形,胞质疏松肝索肿胀明显,肝窦、中央静脉狭窄,2 000 mg/L硝酸钐染毒细胞排列紊乱,肝细胞边缘模糊,部分肝细胞有破裂现象;电镜检查显示50 mg/L硝酸钐染毒组细胞核核周隙不均匀,核膜曲折不平。线粒体数量减少,粗面内质网增生,有脱颗粒现象,偶见高电子密度致密体,500 mg/L硝酸钐染毒组肝细胞细胞器数量减少,结构模糊,核周粗面内质网消失,糖原颗粒减少,胞质见有多量电子密度高,圆形或椭圆形,大小不等的致密体,2 000 mg/L硝酸钐染毒组细胞核电子密度降低,胞质中有较多密度较高,体积较小致密体,核旁可见脂滴,线粒体电子密度较低,结构不清晰,内质网扩张。结论 高剂量硝酸钐对肝结构具有损害作用,提示肝脏是稀土元素钐作用的靶器官。Abstract: Objective To observe the influence of subchronic samarium exposure on liver tissue structure in mice.Methods Eighty mice were randomly divided in to 5 groups.The mice in control group were exposed to pure water and the mice in experimental group were exposed to 5, 50, 500, and 2 000 mg/L aqueous solution of samarium nitrate.After 90 days treamtent, the mice were killed and their liver were rapidly and accurately weighed and the histological changes of the liver were examined with light microscopy and electronic microscopy.Results Compared with the control group, the body weight of the exposed groups was not obviously different.The liver index of 2000 mg/L samaric nitrate trentment group was remarkably lower than that of the control group.Lightmicroscope inspects showed swelling, mild distortion, and the loose of liver cells, the swelling of hepatic cord, and the narrow of liver sinus and central vein in the mice exposed to 500 mg/L samaric nitrate.The rupture and fuzzy edge of liver cell were observed with light micro scope in the mice with 200 mg/L samaric nitrate exposure.The unevenness of per inuclear gap and karyotheca winding, reduction of mitochondria, hyperpasia of rough endoplasmic reticulum, degranulation, and few high electron density compact body were observed with electronic micro scope in liver cells of 50mg/L samaric nitrate exposure group; the decrease and fuzzy structure of organells, disappearance of rough endopla smic reticulum around the nucleus, decrease of glycogen granule, and some high electron density compact body were observed in liver cells of 500 mg/L group.The decreased electron density of nucleus, many dense bodies with high density and small volume, fat drops around the nucleus,low electron density and unstructured mitochodria, and the expansion of endoplasmic reticulum were observed in liver cells of the mice with 2000 mg/L sam aric nitrate exposure.Conclusion High dose samaric nitrate has certain harm effects on livers structure suggesting the liver is the targe to rgan of sam arium.
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