Abstract: ObjectiveTo investigate the expression and significance of heat shock protein 27(HSP27)in lung squamous carcinoma tissues.MethodsThe expression of HSP27 was detected with real-time quantitative PCR(RQ-PCR) and western blot in 22 lung squamous carcinoma tissues and corresponding adjacent normal tissues with different topographylymphnode-metastasis(TNM)stages and differentiated degree.Then the relationship between the expression of HSP27 and pathological character of the tumor was analyzed.ResultsThere was no significant difference in expression level of HSP27 mRNA between lung squamous carcinoma tissues and the adjacent normal tissues.The expression level of HSP27 protein was higher in lung squamous carcinoma tissues than in adjacent normal tissues.HSP27 expression was higher in poorly differentiated tissues than in moderate and high differentiated tissues in lung squamous cell carcinoma(P<0.05).There was no significant difference in the expression of HSP27 protein between tumor tissues of different TNM stage(P>0.05).ConclusionThe expression of HSP27 protein increases in lung squamous cell carcinoma which might play a part in the development of carcinogenesis.