Abstract: Objective To investigate the effect of alumen in food on the activity of ATPase and the contents of Ca and Mg in the cerebrum of rats.Methods Thirty-two Wistar rats were divided into 4 groups at random by weight.The activity of Na⁺K⁺-ATPase,Ca²⁺-ATPase and Mg²⁺-ATPase and contents of Al,Ca and Mg in cerebrum tissues were determined.The pathological changes of the cerebrum were observed.Results The contents of Al of low,moderate,and high dose alu-men group were 2.19±0.39,2.48±0.33,and 3.52±0.41 μg/g and significant higher than that of the negative control group(0.52±0.12 μg/g).The magnesium concentration and the activity of Mg²⁺-ATPase of the high alumen group was lower than that of the negative control group.The activity of Na⁺K⁺-ATPase of low,moderate,and high dose alumen group were 2.87±0.26,1.91±0.11,and 0.24±0.10 μmolPi/mgprot/hour and lower than that of the negative control group(3.28±0.35 μmolPi/mgprot/hour).Pathological study showed intercellular substance edema and cytoplasm porosity of the the brain cells of the rats with alumen exposure..Conclusion Alumen can change normal structure of cell,influence the activity of ATPase and contents of Ca and Mg in the cerebrum of rats.