Abstract: Objective To study sterilizing effect of neutral electrolyzed oxidizing water(NEOW) on Bacillus subtilis var.niger(ATCC 9372) and Escherichia coli(8099).Methods Suspension quantitative germicidal test was adopted.The NEOW was made in electrolysis cell.The sterilizing effects of NEOW,electrolyzed oxidizing water(EOW),NaClO and HCl on Bacillus subtilis var.niger and Escherichia coli were compared.Results The sterilization rate of NEOW was the same to that of EOW.The sterilization logarithm value of NEOW for Escherichia coli was 8.34 logcfu·mL^-1 in 1min and 7.76 logcfu·mL^-1 in 20min for Bacillus subtilis var.niger.But the sterilization logarithm value for Bacillus subtilis var.niger of NaClO with the same available chlorine concentration value as NEOW was 5.76 logcfu·mL^-1 and that of HCl with the same pH value as EOW was 3.06 logcfu·mL^-1 in 20min.In addition,with the increase of available chlorine concentration and time,the sterilization rate increased.Conclusion Sterilizing effect of NEOW is efficient and quick.NEOW could be applied widely in medicine and food industry because of its netural pH.