Abstract: Objective To assess the influence of gene-environment and gene-gene interactions on longevity in Bama county.Methods Both gene-environment and gene-gene interactions among 189 longevous individuals(≥90 years old) from both longevity and non-longevity regions were evaluated according to case-only approach using cross-product odd ratio(COR).Blood samples were collected from all subjects.Alleles and genotypes of 23 loci,such as CSF1PO A9,were analyzed.Results Significant negative interactions between longevity region and D7S820 A12(COR=0.353,95% confidence intervalCI:0.142-0.882,χ²=5.287,P=0.021),longevity region and TH01 A10(COR=0.111,95% CI:0.038-0.328,χ²=17.439,P=0.000),longevity region and TH01 A9-10(COR=0.062,95% CI:0.014-0.283,χ²=21.009,P=0.001) were showed when ten alleles and thirteen genotypes were analyzed.There was no significant gene-gene interaction between D7S820 A12 and TH01 A10(COR=1.536,95% CI:0.543-4.345,χ²=0.279,P=0.597).Conclusion The results suggest that the population from longevity region carrying the alleles of D7S820 A12,TH01 A10,and TH01 A9-10 genotype may reduce longevity.Gene-enviroment interactions between longevity region and genes may influence longevity in Bama,which is probably one of the reasons that there is a nonuniform distribution of longevous population in Bama.