Abstract: Objective To study the cognition,attitude,and demand on premarital medical examination of among college studetns in Guangxi Zhuang Autonomous Region for making effective publicity strategy and improvement of premarital medical examination.Methods A self-administered anonymous questionnaire survey was conducted in 7 universities in Guangxi.Totally 2 154 college students were selected by using stratified cluster random sampling.Results Totally 2 072 eligible questionnaires were collected.The proportion of the students with good,general,litlle,and no knowledge of premarital medical examination was 7.4%,35.9%,53.9%,and 2.8%,respectively.The female,senior student,major in medicine,without a girlfriend/boyfriend had higher cognition of premarital medical examination(P<0.05 for all).There were 65.1% of the students reporting the will to take premarital medical examination in the future and 46.8% reporting the will to take the examination free of charge.There were 71.7% of the students considering the fee for the examination being less than 200 RMB yuan.Conclusion The general cognition of premartial medical examination among college students is at a lowlevel,especially among the male,the students of lower grade or with the major in literature and history.