Abstract: Objective To examine the relationship between perceived stress and subjective well-being and to provide basis for promoting mental health among middle school students.Methods Questionnaires of subjective well-being and Perceived Stress Scale were used to survey 1 000 middle school students from 4 middle schools in Xinjiang Uygur Autonomous Region.Results The scores of girls were significantly higher than those of boys in perceived stress(t=11.28,P=0.001),but lower in subjective well-being(t=2.00,P=0.045),and there were differences in perceived stress and subjective well-being amomg the students of different grade(F=3.54,P=0.003; F=5.63,P=0.000).Perceived stress showed positive correlation with life satisfaction and interests(r=0.072,P < 0.05)and negative correlation with health concerns and relaxation of tension(r=-0.102,r=-0.112,P < 0.05).Foresee showed negative correlation with subjective well-being and all factors(except health concerns)(r=-0.559-0.374,P < 0.01).Control feeling showed negative correlation with health worry(r=-0.073,P < 0.05)and positive correlation with subjective well-being and all factors(r=0.161-0.291,P < 0.05).Overload showed positive correlation with subjective well-being and all factors(except health concerns)(r=0.174-0.293,P < 0.05).Perceived stress had obvious negative prediction to subjective welling(Beta=-0.184--0.673,t=-2.216--8.626,P < 0.05).Conclusion Perceived stress correlates closely with subjective well-being among Uygur middle school students.