Abstract: Objective To study the compliance of pre-exposure prophylaxis(PrEP)medication and its influencing factor among Uighur HIV-negative spouses of HIV-positive individuals and to provide clinical basis for the application of AIDS prevention drugs.Methods Intervention treatment was oral uptake of tenofovir(300mg)combined with emtricitabine(200mg).A total of 114 Uighur subjects were randomly divided into two groups and once-a-day and twice-a-weektreatment were given to each group for 7 months with a follow-up of every 4-week to knowthe compliance of the subjects.Results The compliance rate of PrEP reached 74.56%(85/114).Single-factor analysis revealed that the proportion of high HIV infective possibility in the compliance group and non-compliance group was 57.65%(49/85)and 13.79%(4/29),with a significant difference between the two groups(Z=-2.77,P < 0.001).The adherence rate of PrEP was 64.71%(55/85)and 27.59%(8/29)for compliance and non-compliance group when their spouse disagreed to the PrEP,and the possible adherence rate was 16.47%(14/85)and 27.59%(8/29)for the two groups,with a significant difference between the two groups(Z=-3.51,P < 0.001).Logistic regression analyses indicated that higher perceived infective possibility(odds ratioOR=4.59,95% confidence intervalCI:1.46-14.36)and higher willingnes of adherence to PrEP when their spouse disagreeing PrEP(OR=3.78,95% CI:1.25-11.42)were factors for the improvement of PrEP medicine compliance.Conclusion PrEP medication promotion is applicable among HIV-negative spouses of HIV-positive individuals.The HIV-positive individuals'attitude to PrEP of their spouses and the risk of HIV-infection are major factors influencing medication compliance.