Abstract: Objective To evaluate antifungal effects of 7 terpenes against Aspergillus flavus(A.flavus)and to provide basis for development of antimicrobial against A.flavus.Methods The anti-A.flavus effects of the terpenes were investigated at the concentrations of 1 μL,2 μL,4 μL,and 8 μL with gaseous diffusion methods.Results All treatments had inhibitory effect on hyphal growth,with the exception of 1 μL farnesen,1 μL β-ocimene,and 1 μL α-pinene.Myrcene at the concentrations of 1 μL,2 μL and 4 μL,β-ocimene at 1 μL,and terpinene at 1 μL were lack of inhibition on spore germination.The terpenes at other concentrations could inhibit spore germination significantly.The antifungal effect of linalool was the strongest with an inhibitory rates on hyphal growth and spore germination of 76.53% and 86.32% at the concentration of 8 μl/L.Conclusion The results indicate that the seven terpenes could inhibit A.flavus at different extent,and the effects of linalool is the best.