Abstract: Objective To evaluate the effects of hepatitis B(HB)vaccine inoculation and health education in undergraduates of Gansu province.Methods Questionnaire surveys on awareness rate and attitude about hepatitis B and the immunization coverage rate of HB vaccine were conducted before and after implementation of the projects in Gansu province.Multiple stratified cluster sampling method was used in the survey.Results After the implementation of the projects,the HB virus vaccine full-course coverage rate among the students was improved from 27.95% to 97.14% compared with the baseline survey.The awareness rate of undergraduates about transmission way of hepatitis B was improved from 31.35% to 68.18%(P < 0.001).The awareness rate about other hepatitis B knowledge was increased from 63.92%-85.05% to 84.32%-90.73%(P < 0.001).The number of the undergraduates who thought hepatitis B being a serious illness was increased from 74.77% to 88.42%.The ratio of students trying to avoid contacting with HB virus carriers was decreased from 8.69% to 1.82%.And the ratio of students not minding eating together with HB virus carrier was increased from 29.27% to 64.57%(P < 0.001).Conclusion Through implementation the project,the awareness of undergraduates about hepatitis B is obviously improved and the discrimination against HB virus carrier in the undergraduates decreased.The HB virus vaccines full-course coverage rates among the students are significantly increased.