Abstract: ObjectiveTo evaluate transcription levels of malaria invasion related molecules of morozolite surface protein-1(MSP-1),apical membrane antigen-1(AMA-1)and 235 kDa rhoptry protein(Py 235)after short-time nitric oxide (NO)donor(NOC 5)treatment.MethodsFemale BALB/c mice infected with P.yoelii 17XL were inoculated with NOC 5 by intraperitoneal injection.The mature parasite schizonts was enriched and total RNA was extracted from schizonts.The transcription levels of MSP-1,AMA-1 and Py 235 were detected with real-time PCR.Results The transcription levels of MSP-1 and AMA-1 were down-regulated.As a virulence marker,the gene 5054 of Py 235 showed low transcription level.ConclusionThe important invasion related molecules of MSP-1 and AMA-1 were inhibited after NO treatment.The parasites adjusted its transcriptional pattern and adapted to the host immune environment.