Abstract: ObjectiveTo compare the difference in sre homology 2 domain(SH2-B)βexpression in hypothalamus and lung between diet-induced obese mice and normal mice(NC),and to explore the association of SH2-Bβand obesity and inflammation.MethodsForty-five C57BL/6 mice(male)aged 6 weeks were randomized to a standard rodent chow (NC,n=20)or to a high fat diet chow(HFD,n=25).Western blot was used to detect SH2-Bβexpression in hypothalamus and immunohistory was adopted to detect SH2-Bβexpression in lung.By means of animal lung function analysis system,the changes of the airway resistance was investigated.Results Immunohistochemistry detection demonstrated that more imflammatory cells were in the lung in obese mice than NC group,and the mean optic density(MOD) (0.685±0.025)of SH2-Bβpositive substance in the airway epithelium and imflammatory cells of the obese mice was much higher than those of NC group(0.127±0.019)(P<0.01).Western blot results demonstrated that the mean ratio of SH2-BβMOD toβ-actin MOD in hypothalamus was 0.686±0.056 in obese mice,much lower than that of in NC group (2.487±0.204)(P<0.01).Airway resistance was increased significantly in obese mice compared to that of NC group.ConclusionHigh fat diet may decrease SH2-Bβexpression in hypothalamus and induced obesity.SH2-Bβexpression in airway epithelium and imflammatory cells increases and results in higher airway resistance.