Abstract: Objective To evaluate the validity and reliability of World Health Organization Quality of Life Scale-Abbreviatd Version(WHOQOL-BREF) among nurses with night shift.Methods Cluster random sampling method was used to select one grade A class III-hospital in Tianjin city and all the nurses with night shift in the hospital (385 nurses in all) were investigated with WHOQOL-BREF.Split-half reliability,Cronbach's α coefficient and the correlation coefficient between score of domain and total score were used to evaluate the reliability of the scale; content validity,discriminated validity,and construct validity were used to evaluate the validity of the scale.Results Split-half reliability of WHOQOL-BREF was 0.77 and the overall Cronbach's coefficients of scale was 0.91.The split-half reliability of physical,psychological,social,and environment domain was 0.614-0.723,and the Cronbach's α coefficient was 0.722-0.835(P<0.001).WHOQOL-BREF showed good internal consistency.The correlations between each item and its domain were greater than 0.50(P<0.001) and they were greater than the correlation between the item and other domains,except for the physical domain,which demonstrated that WHOQOL-BREF had good content validity and discriminatory validity.The results of factor analyses showed that six principal components extracted from the items representing all domains of the scale and the cumulative variance was 60.76%,which demonstrated the scale had good construct validity.The nurses with different age,work experience,education level had different scores in physical and psychological domain.Conclusion WHOQOL-BREF has good reliability and validity and is applicable for the evaluation of quality of life among nurses with night shift.