Abstract: ObjectiveTo explore the acceptability of female condom among the female sex workers in the entertainment establishments in developed urban area,Hainan province.MethodsQuota sampling was adopted to recruit female sex workers aged more than 16 years and having commercial sex within latest 30 days at high-,moderate-,and low- grade entertainment establishments and a cross-sectional study was conducted before and after the intervention.ResultsTotally 152 and 161 eligible questionnaires were collected in the baseline survey and the post-intervention survey.There were no significant differences in demographic characteristics between the participants of the two surveys except in the registered residence of the participants(χ²=14.102,P<0.001).In the baseline survey,there were only 7 people knowing about female condom,one person seeing it and no one using it.In the post-intervention survey,there were 139 people knowing about the female condom,120 people seeing it and 41 people using it.All the participants,both in the baseline and the post-intervention survey,had high acceptability of the anti-diseases(95.4%,93.8%),anti-sexually transmitted diseases(93.4%,91.9%),and anti-pregnancy(92.8%,92.6%) function of female condom.But the participants reported negative attitude towards the appearance,application method and feeling of the female condom.ConclusionFemale condom could be accepted by the female sex workers in the entertainment establishments in the city,but its appearance,touch feeling and application method need to be improved.