Abstract:
Objective To observe the effects of microcystin-LR(MCLR) on methyltransferase activity in mice liver.
Methods Eighty mice were randomly divided into 0,5,11 and 21 μg/kg group according to the dose of MCLR.MCLR was administered for 10 and 20 days,respectively.The liver function and DNA methyltransferase activity were determined.Morphological changes of livers were observed.
Results Compared with the 0 μg/kg group,the levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) in serum of the 5 μg/kg group were not obviously different after the exposure of 11 days.The levels of ALT,AST,and ALP in 20 μg/kg group were statistically significantly higher than that of 0 μg/kg group(
P<0.05) after the exposure of 11 days.The levels of ALT and AST in 5,10,20 μg/kg group were statistically significantly higher than that of 0 μg/kg group(
P<0.05) after the exposure of 21 days.The DNA methyltransferase activity in 5,10,and 20 μg/kg group exposed for 11 days were 89 135.7±14 334.7,92 645.8±15 962.3,and 70 481.3±15 621.25 relative fluorescence unit(RFU)/h/mg,respectively,with statistical differences compared with that of 0 μg/kg group(
P<0.05).The DNA methyltransferase activity in 5,10,and 21 μg/kg group exposed for 20 days were 59 115.6±9 884.6,83 310.7±15 066.3,and 86 612.5±20 572.8 RFU/h/mg,respectively,with statistical differences compared with that of 0 μg/kg group(
P<0.05).
ConclusionMCLR could induce the increases of ALT,AST,ALP levels and up-regulation of methyltransferase activity in mice liver.