Abstract: Objective To investigate the effect of different dose of zinc on insulin sensitivity in rats fed with high sucrose and fat diet.Methods Totally 68 healthy male Sprague-Dawley rats were randomly allocated to two groups:normal diet group(n=29) and high-sucrose-fat diet group(n=39).After 17 weeks,the rats of the two groups were randomly assigned to five groups,each group was administered with distilled water or zinc at dose of 5,10,15 or 30 mg/kg daily by gavage for 7 weeks.Fasting plasma glucose,fasting plasma insulin(FINS) and glycohemoglobin(HbA₁c) were detected.Then the homeostasis model assessment for insulin resistant(HOMA-IR) and insulin sensitiveiy index(ISI) of each group were calculated.Results Compared with normal diet group,the glucose of high-sucrose-fat diet groups was insignificantly different before or after the gavage.FINS and HOMA-IR were significantly increased among high-sucrose-fat diet groups after zinc administration(P<0.05),while the FINS for the group of 10 mg zinc was 13.574± 2.297 μIU/ml and that of 15 mg zinc group was 11.050± 1.654 μIU/ml,both lower than other zinc level groups(P<0.05).ISI were significantly decreased among high-sucrose-fat diet groups after zinc administration,and that for the group with 15 mg zinc gavage was-1.733± 0.07530,significantly lower than that of other groups(P<0.05).Conclusion Zinc could at some degree improve insulin sensitivity in rats fed with high-sucrose-fat diet,depending on the dose.