Abstract: ObjectiveTo evaluate disinfection efficacy of conventional disinfectants against human immunodeficiency virus(HIV). MethodsTwo methods(nucleic acid quantification and cell culture)were used in testing of disinfectant against HIV in blood or plasma. ResultsThe nucleic acid quantification test showed that the log inactivation value for HIV in plasma exceeded 4.00 in the case of HIV exposed to available chlorine of 3 000 mg/L for 5 min,available iodine of 100 mg/L for 1 min or 75% alcohol for 1 min.But for the exposure of peracetic acid 5 000 mg/L for 1 min,the log inactivation value did not exceed 2.00.For the HIV in blood,the concentration of avaiailable chlorine and iodine should be increased for effective disinfection.The cell culture test showed that the log inactivation value exceeded 4.00 in case of HIV exposed to 100 mg/L available chlorine or peracetic acid for 3 min,available iodine 100 mg/L for 1 min,75% alcohol for 30 s,and 0.05% glutataldehyde for 5 min. ConclusionThere are differences in disinfection effect between the two methods.As an indirect indicator of inactivation,nucleic acid quantifecation could be used in the quick screening test while culture is a direct indicator which could be considered as the ultimate determination.