Abstract: ObjectiveTo explore the effect of glycogen synthase kinase-3β(GSK-3β)overexpression on cardiac stem cells(CSCs)based therapy in myocardial infarction rats. MethodsRat model of cardiac infarction was established.CSCs were isolated and lentivirus-based transfection of GSK-3β or LacZ were performed.Cardiac functions of the two groups were measured and compared;collagen contents in ventricle were also measured and compared. ResultsCompared with CSCs-LacZ transplantation group,rats receiving CSC-GSK-3β showed an increased left ventricular ejection fraction(LVEF)and a decreased left ventricular internal dimension at end diastole(LVEDd).Ventricular collagen contents were also reduced in CSCs-GSK-3β group. ConclusionOverexpression of GSK-3β enhances cardiac stem cell based therapy in myocardial infarction rats.