Abstract: Objective To establish an accurate,rapid,and sensitive method for simultaneous detection of three quinolones(QNs) including ofloxacin(OFL),ciprofloxacin(CIP),and enrofloxacin(ENR)residues in animal edible products.Methods The residues in the samples were extracted by acidified acetonitrile(ACN)and ultrasonic wave device,and then the impurities were removed by liquid-liquid extraction(LLE)with hexane.After concentrated,the residues were dissolved by ACN-water(8:2)and then determined with high-performance liquid chromatograply-ultraviolate-visible detector(HPLC-UVD).The mobile phase was ACN and sodium dihydrogen phosphate solution(0.01mol/L,pH=4.0);gradient elution was used and the detection wavelength was 279 nm.Results OFL,CIP and ENR were seperated succssfully and the chromatographic peaks were sharp and symmetrical.The correlation coefficients were all above 0.999 6.The relative standard deviations of OFL,CIP,and ENR were all below 5%,with good reproducibilities.The recovery rates were all more than 85%.The limit of determination(LOD)of OFL,CIP,and ENR was 2.7×10^-3 μg/ml,1.4×10^-3 μg/ml,and 6.5×10^-4 μg/ml,respectively.ConclusionThe method established is suitable for rapid,accurate,and simultaneous detection of OFL,CIP,and ENR in animal edible prodults.