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染铅大鼠铁过载致神经元损伤特殊染色法分析

杜桂花 陈英 黎砚书 焦欢 周繁坤 祝高春 冯昶 范广勤

杜桂花, 陈英, 黎砚书, 焦欢, 周繁坤, 祝高春, 冯昶, 范广勤. 染铅大鼠铁过载致神经元损伤特殊染色法分析[J]. 中国公共卫生, 2014, 30(2): 197-200. doi: 10.11847/zgggws2014-30-02-21
引用本文: 杜桂花, 陈英, 黎砚书, 焦欢, 周繁坤, 祝高春, 冯昶, 范广勤. 染铅大鼠铁过载致神经元损伤特殊染色法分析[J]. 中国公共卫生, 2014, 30(2): 197-200. doi: 10.11847/zgggws2014-30-02-21
DU Gui-hua, CHEN Ying, LI Yan-shu.et al, . Quantitative analysis of neuron damage caused by iron overload in lead exposure rats with special staining image method[J]. Chinese Journal of Public Health, 2014, 30(2): 197-200. doi: 10.11847/zgggws2014-30-02-21
Citation: DU Gui-hua, CHEN Ying, LI Yan-shu.et al, . Quantitative analysis of neuron damage caused by iron overload in lead exposure rats with special staining image method[J]. Chinese Journal of Public Health, 2014, 30(2): 197-200. doi: 10.11847/zgggws2014-30-02-21

染铅大鼠铁过载致神经元损伤特殊染色法分析

doi: 10.11847/zgggws2014-30-02-21
基金项目: 

国家自然科学基金(30960324;81273120;21267017)

详细信息
    作者简介:

    杜桂花(1988-),女,福建三明人,硕士在读,研究方向:重金属的神经毒性。

    通信作者:

    范广勤,E-mail:fanfgq@126.com

  • 中图分类号: R995

Quantitative analysis of neuron damage caused by iron overload in lead exposure rats with special staining image method

  • 摘要: 目的探讨长期铅暴露大鼠铁过载对神经元的损伤。方法SD大鼠母代与子代分别设对照、低、高剂量铅暴露组(母代:800、1 500 mg/L,子代:300、900 mg/L 乙酸铅饮水方式染毒)连续70周;电感耦合等离子体发射光谱仪(ICP-AES)测血液和海马元素铅及铁,同部位海马石蜡切片分别行铁染色及硫堇染色并进行图片融合。结果染铅后,大鼠血和海马铅及海马铁含量均增加(P<0.01);低、高剂量铅暴露组子代大鼠海马CA3区铁沉积IOD值分别为(5 930.71±2 517.68)和(1 1382.43±2 551.14),均高于对照组的(3 786.78±1 256.37),差异有统计学意义(P<0.01);低、高剂量铅暴露组子代大鼠海马CA1区的神经元数量分别为(70.71±11.80)、(70.37±17.53)个/视野,均低于对照组的(83.71±12.60)个/视野,差异有统计学意义(P<0.01);图像融合显示,染铅大鼠神经元内铁沉积增加而神经元数量及层数减少,损伤加重。结论基于铁染色和硫堇染色图像融合可直观地观察铅暴露大鼠铁过载部位神经元损伤。
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出版历程
  • 接收日期:  2013-02-22
  • 刊出日期:  2014-02-10

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