Abstract: Objective To study candidate reference materials,processes route and quality control indexes for the detection of bio-waste oil.Methods Using the most common and convenient raw material and the most probable technical processes,reference material for bio-waste oil detection was screened taking the appearances,gas taste,color,water and volatiles,acid value as quality control indexes.Results Kitchen waste oils were collected and candidates of bio-waste oil reference materials were prepared by the processes of distilling deodorization,alkali refining deacidification and adsorption decolorization.These candidates have the index values of color(1",R)between 4.0-5.1,water and volatile between 0.02%-0.05%,and acid between 5.60-6.30 mg/g.Conclusion The candidates could be used to prepare reference material for detection of specific substance in bio-waste oils with good specificity,sensitivity and detection limit.