Abstract: Objective To establish an insulin resistance model in HepG2 cells and to observe the effect of mangiferin on insulin resistance in vitro.Methods With insulin-induced HepG2 cells,insulin resistance model was established and the effects of mangiferin on insulin resistance,glucose metabolism,and lipid oxidation in HepG2 cells were detected.Results Compared with the control group,glucose level(6.201±0.049 mmol/L)elevated in the insulin resistance model group and glycogen content(0.722±0.0071 μg/μgprot)decreased;malodialdehyde(MDA)content(3.224±0.203 nmol/mgprot)increased and superoxide dismutase(SOD)activity(48.406±7.183 U/mgprot)declined.Compared with the model group,glucose level(5.183±0.161 mmol/L)reduced;glycogen content(1.122±0.115 μg/μgprot)significantly increased;MDA content(1.511±0.374 nmol/mgprot)declined;and SOD activity(79.891±9.926 U/mgprot)increasesd in HepG2 cells treated with mangiferin(1 000 g/ml),with a dose-response relationship.Mangiferin had no obvious effect on glucose metabolism in normal hepG2 cells.Conclusion Mangiferin could improve sugar metabolism,which may be pertinent to promote glycogen synthesis and reduce lipid peroxide formation,improve the activity of antioxidant enzymes in insulin resistance HepG2 cell model.