Effects of leucine on nonalcoholic fatty liver disease induced by high fat diet in rats
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摘要: 目的 观察亮氨酸对高脂大鼠非酒精性脂肪性肝病(NAFLD)的影响及机制。方法 40只SD大鼠(60%高脂饲料喂养)随机分为4组,高脂组及1.5%、3.0%、4.5%亮氨酸组,连续干预16周,检测血清丙氨酸转移酶(ALT),门冬氨酸转移酶(AST)和总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,取肝脏观察病理改变,检测肝组织总抗氧化能力(T-AOC)、总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)水平。结果 高脂组大鼠肝脏呈现明显脂肪变性;与高脂组比较,亮氨酸各剂量组大鼠ALT、AST的水平明显降低(P<0.01);与高脂组比较,3.0%、4.5%亮氨酸组大鼠血清TC水平[分别为(2.35±0.30)、(2.60±0.57)mmol/L]明显下降,肝脏T-SOD[分别为(285.99±53.53)、(272.47±65.02)U/mgprot]和GSH-Px[分别为(506.02±140.34)、(483.44±131.44)U/mgprot]活性明显升高(P<0.01),4.5%亮氨酸组大鼠肝脏MDA含量[(0.47±0.14)nmol/mgprot]明显降低(P<0.01)。结论 亮氨酸能够改善高脂膳食诱导的大鼠NAFLD,其机制可能与改善脂代谢、增强机体抗氧化能力有关。
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关键词:
- 亮氨酸 /
- 非酒精性脂肪性肝病(NAFLD) /
- 脂代谢 /
- 抗氧化能力
Abstract: Objective To investigate the effects and mechanism of leucine on nonalcoholic fatty liver disease(NAFLD) induced by high fat diet in rats.Methods Forty male Sprague-Dawley rats fed with 60% high fat diet were randomly divided into 4 groups:one high-fat diet group and three leucine groups with 1.5%,3%,and 4.5% leucine in the high-fat diet,respectively.At the end of 16 weeks' treatment,the serum alanine aminotransferase(ALT),aspartate transferase(AST),total cholesterol(TC),triglycerides(TG),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),liver hepatic total anti-oxidation capacity(T-AOC),total superoxide dismutase(T-SOD),reduced glutathione hormone(GSH-Px),and malondialdehyde(MDA) were measured.The pathological changes in the livers of the rats were also observed.Results Obvious fatty degeneration of the livers was observed in the rats of the high-fat diet group.Serum levels of ALT,AST in leucine groups were signi-ficantly lower than those of the high-fat diet group.The serum TC in 3.0% and 4.5% leucine groups were 2.35±0.30 and 2.60±0.57 mmol/L,which were signifi-cantly lower than those of the high-fat diet group.Compared to those of the high-fat diet group,significantly higher T-SOD(285.99±53.53,272.47±65.02 vs.208.24±63.57 U/mg.prot,P<0.01) and GSH-PX(506.02±140.34,483.44±131.44 vs.209.50±80.38 U/mg.prot,P<0.01) were detected in the liver of the rats of 1.5% and 3.0% leucine groups,but significantly lower MDA(0.47±0.14 vs.1.04±0.43 nmol/mg.prot,P<0.01) was detected in 4.5% leucine group.Conclusion Leucine improves NAFLD induced by high fat diet in rats and the effect is related to improving lipid metabolism and antioxidant capacity.-
Key words:
- leucine /
- nonalcoholic fatty liver disease /
- lipid metabolism /
- antioxidant
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