Measurement of bone growth factor and its clinical significance in patients with humeral shortness
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摘要: 目的 检测肱骨短小症患者及正常对照者血清中骨形成蛋白-2(BMP-2)等7种骨生长因子,观察2组差异,为探讨肱骨短小症患者的病因提供线索。方法 在西藏怒江流域,取肱骨短小症患者12例及正常对照16人的外周静脉血,分离血清,用ELISA法检测血清中骨生长因子的浓度,将2组骨生长因子的浓度取对数,采用t检验进行比较。结果 病例组BMP-2、BMP-4、BMP-7、转化生长因子-β2(TGF-β2)、成纤维细胞生长因子受体-3(FGFR3)、生长分化因子-5(GDF-5)和成纤维细胞生长因子-23(FGF23)的浓度均值分别为3.35、3.14、568.24、625.09、3.01、3.26 ng/mL和161.33 pg/mL,对照组浓度均值分别为4.61、3.25、2 300.35、1 849.47、5.99、8.09 ng/mL和373.47 pg/mL,病例组BMP-7、FGFR3、GDF-5浓度平均水平低于对照组,差异有统计学意义(P<0.05);相关分析显示,病例组中的BMP-2等7种骨生长因子之间呈高度相关,对照组中BMP-2只与BMP-4、TGF-β2呈高度相关性,而与BMP-7、GDF-5、FGFR3、FGF23相关较弱。结论 肱骨短小症患者可能因为BMP-7、FGFR3、GDF-5 3种骨生长因子浓度发生变化,并通过多种生长因子的相互作用而发病。Abstract: Objective To monitor the levels of seven bone growth factors in humeral shortness patients and normal controls.Methods Peripheral blood of 12 humeral shortness patients and 16 normal controls were collected in Nujiang basin of Tibet.The levels of bone morphogenetic protein-2(BMP-2),bone morphogenetic protein-4(BMP-4),bone morphogenetic protein-7(BMP-7),transforming growth factor-β 2(TGF-β2),fibroblast growth factor receptor-3 (FGFR3),growth differentiation factor-5 (GDF-5),and fibroblast growth factor-23(FGF23) were measured with enzyme-linked immunosorbent assay(ELISA).Results The content of BMP-2,BMP-4,BMP-7,TGF-β2,FGFR3,GDF-5,and FGF23 in humeral shortness patients were 3.35,3.14,568.24,625.09,3.01,3.26 ng/mL,and 161.33 pg/mL,respectively,and those in normal controls were 4.61,3.25,2 300.35,1849.47,5.99,8.09 ng/mL,and 373.47 pg/mL.The contents of BMP-7,FGFR3 and GDF-5 in humeral shortness patients were lower than those of normal controls,and there were significant differences between the two groups.The results of Pearson correlation analysis showed that the seven growth factors were highly correlated in the patient group,while in the normal control group,BMP-2,BMP-4,and TGF-β2 were highly correlated,but the correlations of BMP-7,GDF-5,FGFR3 and FGF23 were weak.Conclusion The causative factor of humeral shortness may be the changes of BMP-7,FGFR3 and GDF-5 and the interactions of various growth factors may affect the mcidence of the disease.
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Key words:
- humeral shortness /
- osteochondropathy /
- osteodysplasty /
- growth factor
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