Abstract: Objective To assess the effectiveness of interventions on knowledge,attitude and practice about drinking driving among general population in Nanjing city of Jiangsu province.Methods Multi-stage stratified cluster sampling was applied to conduct a questionnaire survey among residents aged 18-69 years and living in Nanjing city at least six months both before and after an intervention program for drinking driving in the public.Results Totally 2 290 and 2 245 eligible questionnaires were collected in the two surveys.The awareness rates for the minimum blood alcohol concentration as the criteria of driving under the influence of alcohol(DUI)and driving while intoxicated(DWI),the duration of license suspension for driving a non-commercial and commercial motor vehicle while intoxicated among the participants were 20.2%,14.2%,6.6%,and 6.0% before the intervention and 42.2%,35.5%,11.8%,and 13.7% after the intervention,with significant increases after the intervention(all P<0.001).No significant difference in attitude toward drinking driving among the participants after the intervention.The self-reported drinking driving rate of post-intervention(10.9%)was lower than that of pre-intervention(15.7%)and self-reported drinking driving rate decreased significantly for the participants of living in suburb areas,male,aged ≤40 years,with the education of junior high school or below,and the married(P<0.05 for all).Self-reported witness of police enforcement activity on drinking driving,breath alcohol test on drivers,reading outdoor advertisements,billboards,brochures,and other materials about drinking driving were also increased after the intervention.Conclusion The awareness and behavior about drinking driving were improved among general population after the intervention program and the public maintained a good attitude toward drinking driving