Abstract: ObjectiveTo establish an immortal cell line from Shui and Gelao ethnic populations in Guizhou province and to provide a permanent research material for life science research.MethodsTotally 144 peripheral venous blood samples from Shui ethnic people in Sandu county and 124 from Gelao in Wuchuan county in Guizhou province were collected.B lymphocytes isolated from the blood samples were transformed using Epstein-Barr(EB) virus infection and cyclsoporine A(CyA) treatment.The morphology of the transformed cells was observed and direct counting method was used to make cell growth curve and to calculate cell doubling time of the transformed cells.Passage,frozen storage,and recovery of the transformed cells were performed and culture was conducted to detect mycoplasma contamination.ResultsTotally 138 immoral B lymphocyte cell lines from Shui ethnic group and 115 from Gelao ethnic group were successfully established with the conversion rates of 95.8% and 92.7%,respectively.The transfored B lymphocytes present typical cell deformation,cloning growth characteristics,and normal growth after recovery,without mycoplasma contimination.ConclusionPeripheral B lymphocytes from Shui and Gelao ethnic populations were transformed into immortal cell lines successfully for further researches.