Abstract: ObjectiveTo investigate the results of bacterial culture and drug sensitivity test for clinical specimens collected in the Central Hospital of Chaoyang City during the first half of 2012.MethodsIdentification and drug sensitive test were conducted for bacterial isolations of various clinical specimens from out- and in-patients of the hospital.ResultsTotally 1 024 bacterial strains were isolated from 2 764 clinical specimens,of which,705(68.8%)were gram-negative bacilli and 238(23.2%)were gram-positive cocci.The main gram-negative bacilli isolated were Pseudomonas aeruginosa(20.4% of the all isolated strains),Bauman Acinetobacter(17.5%),Escherichia coli(14.7%),and Klebsiella pneumoniae genus(13.9%) and the major gram-positive cocci were coagulase negative Staphylococcus(12.7%) and Staphylococcus aureus(9.96%),respectively.The gram-negative bacilli isolated were sensitive to amikacin,imipenem,meropenem,cephalosporin cefepime,and piperacillin/tazobactam and the gram-positive cocci were sensitive to minocycline,vancomycin,and rifampin.ConclusionMonitoring on clinically isolated bacteria and their drug resistance is important to rational use of antimicrobial agents and effective control of infection in clinical practices.