Abstract:
ObjectiveTo study the expression of methyl-DNA-binding proteins(MBDs)in mitoxantrone-induced drug-resistance human breast cancer cells(MCF-7)and to analyze the relationship between multidrug resistance and MBDs.
MethodsMitoxantrone at 4 different dosages(0.005,0.01,0.02,and 0.04 μmol/L)was administrated to MCF-7 cells for 15 days;then the expressions of breast cancer resistance protein(BCRP)and MBDs(MBD1,MBD2 and MBD4)in the experimental and control MCF-7 cells were determined with Western blot and compared.
ResultsCompared with those of the control cells,the BCRP expression gradually increased with the increment of mitoxantrone,while the expressions of MBDs(especially MBD4)decreased in MCF-7 cells,with the MBD4 expression levels of 0.910±0.049,0.835±0.065,0.708±0.082,and 0.660±0.145(all
P<0.05).
ConclusionMBDs expression,especially MBD4,in MCF-7 cells decreases after exposed to mitoxantrone continuously and the decrease may be related to the formation of drug resistance.