Abstract:
Objective To investigate the effect of oxaliplatin on expressions of nuclear factor κB(NF-κB)and signal transducer and activator of transcription(STAT3)in human colon cancer cells(SW620).
Methods SW620 cells in logarithmic growth period were cultured
in vitro and then treated with different concentrations of oxaliplatin(40,80,160 μg/ml),and a vehicle control group was established.After the treatment(72 hours),3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was applied to assess oxaliplatin-mediated anti-proliferative effect on SW620 cells.Hoechst 33258 staining was used for observing morphological changes of the cells.Meanwhile,Western blot analysis was applied to determine endogenous protein expressions of NF-κB and STAT3 in SW620 cells.
Results Compared with the control group,oxaliplatin administration at different dosages significantly inhibited the proliferation of SW620 cells,with the inhibition rates of 66.19±5.93%,86.92±7.85%,and 96.85±8.86%(
P<0.05 or
P<0.01),and promoted apoptosis of the cells,with the increased number of apoptosic cells of 9.46±1.05,18.57±2.16,and 27.29±3.48/per microscopic field(
P<0.01 for all).In addition,oxaliplatin effectively down-regulated endogenous NF-κB and STAT3 protein expressions in SW620 cells,with the NF-κB expression level of 0.674±0.016,0.435±0.012, and 0.212±0.009 and STAT3 expression level of 0.485±0.015, 0.264±0.008,and 0.113±0.004(all
P<0.01).
Conclusion Oxaliplatin could inhibit proliferation of SW620 cells and the inhibitive effect may be due to the decreased expressions of NF-κB and STAT3 in SW620 cells.