Abstract: Objective To study antibiotic resistance of Vibrio cholerae strains isolated in Guangdong province between 2009 and 2014 and to provide references for prevention and control of cholera.Methods Totally 253Vibrio cholera isolates were tested for the resistance to 12 selected antibiotics by the Kirby-Bauer method.Results Among the 253 isolates,189 strains belong to O1 serogroup(72 Ogawa serotype and 117 Inaba serotype);26 strains belong to O139 serogroup and 38 strains are non-O1 and non-O139 serogroups.Antibiotic susceptibility analysis showed that all the isolated strains were sensitive to cefotaxime and ceftriaxone and the sensitive rates of the isolated strains to norfloxacin,ciprofloxacin,and amikacin were 98.8%,98.4%,and 98.0%,respectively.The resistant rate of isolated Vibrio cholerae strains to nalidixic acid,tetracycline,doxycycline,trimethoprim-sulfamethoxazole,ampicillin,amoxicillin,and chloramphenicol differed significantly among the strains of three different serogroups(P<0.05).Multidrug resistance analysis showed that 37.5% of the strains isolated were resistant to three or more tested antibiotics,of which,12 strains of Inaba serotype were resistant to six or more antibiotics,while only 3 strains of Ogawa serotype and one strain of non-O1 and non-O139 serogroups were resistant to six drugs,2 strains of O139 serogroup were resistant to four drugs.Conclusion Vibrio cholerae stains isolated in Guangdong province are high sensitivity to cefotaxime,ceftriaxone,norfloxacin,ciprofloxacin,and amikacin.But the incidence of non-O1 and non-O139Vibrio cholerae infection increased and some multiple antibiotic resistant strains were detected,suggesting antibiotic resistance surveillance on Vibrio cholerae stains needs to be strengthened.