职业接触者尿中汞微波消解-原子荧光法测定

Determination of urinary mercury among workers with occupational exposure by atomic fluorescence spectrometry and microwave digestion

摘要: 目的建立职业汞接触者尿汞水平的原子荧光测定法。方法用微波消解法对尿样进行前处理,处理后无需赶酸,用5%硝酸溶液做载液,在灯电流15 mA,负高压240 V,原子化高度8 mm,载气流量(Ar气)400 mL/min,屏蔽气流量1000 mL/min条件下,用0.5 g/L硼氢化钾溶液作还原剂做工作曲线测定职业接触者尿中汞浓度。结果该方法在0.01~10.00μg/L范围内具有良好线性关系,r=0.9996,检出限为0.01μg/L,回收率100.0%~107.8%,相对标准偏差(RSD)为2.07%~4.26%。结论该方法可作为职业汞接触者体内汞接触水平的检测方法。

Abstract: Objective To establish a method to detect urinary mercury among workers with occupational exposure by atomic fluorescence spectrometry(AFS) and microwave digestion.Methods The urine specimens from workers with occupational mercury exposure were treated with microwave digestion and then the mercury in the specimens was determined with hydride generation AFS.Results The method showed a good linearity in the range of mercury concentration of 0.01-10μg/L(r=0.9996) and a detection limit of 0.01μg/L;the recovery rates of the method ranged from 100.0% to 107.8%,with the relative standard deviations of 2.07%-4.26%.Conclusion The method could be used to monitor mercury level in vivo among workers with occupational exposure.