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蔡特, 王立志, 张开翼. 大连沿海贝类产品副溶血性弧菌基因检测[J]. 中国公共卫生, 2015, 31(12): 1674-1676. DOI: 10.11847/zgggws2015-31-12-45
引用本文: 蔡特, 王立志, 张开翼. 大连沿海贝类产品副溶血性弧菌基因检测[J]. 中国公共卫生, 2015, 31(12): 1674-1676. DOI: 10.11847/zgggws2015-31-12-45
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CAI Te, WANG Li-zhi, ZHANG Kai-yi. Detection and toxic gene analysis of Vbrio parahaemolyticus in seashells from coastal area of Dalian city[J]. Chinese Journal of Public Health, 2015, 31(12): 1674-1676. DOI: 10.11847/zgggws2015-31-12-45
Citation: CAI Te, WANG Li-zhi, ZHANG Kai-yi. Detection and toxic gene analysis of Vbrio parahaemolyticus in seashells from coastal area of Dalian city[J]. Chinese Journal of Public Health, 2015, 31(12): 1674-1676. DOI: 10.11847/zgggws2015-31-12-45
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大连沿海贝类产品副溶血性弧菌基因检测

Detection and toxic gene analysis of Vbrio parahaemolyticus in seashells from coastal area of Dalian city

    摘要
  • 摘要: 目的 了解辽宁大连沿海地区贝类产品中副溶血性弧菌的污染状况及3种毒力基因的携带情况。方法 采集大连周边海域养殖场及农贸市场132份贝类样品,用实时荧光PCR检测方法进行副溶血性弧菌标靶基因tl基因检测,同时检测tdh、trh和orf8毒力基因并进行分析。结果 在132份贝类样品中,检测出携带有副溶血性弧菌标靶基因43份,检出率为32.6%(43/132),其中携带有tdh基因阳性2份4.7%(2/43),trh基因阳性2份4.7%(2/43), orf8基因阳性1份2.3%(1/43),同时携带有tdh、orf8基因阳性1份2.3%(1/43),同时携带tdh、trh基因阳性16份37.2%(16/43);牡蛎中副溶血性弧菌标靶基因tl检出率为28.8%(23/80),毒力基因同时携带10株。结论 大连贝类海产品中携带副溶血性弧菌情况比较严重。

     

    Abstract: Objective To evaluate the contamination status of Vibrio parahaemolyticus(Vp)and 3 virulence genes in seashells from coastal area of Dalian city.Methods A total of 132 fresh seashells samples were collected from retail markets and oyster farms in Dalian municipality and detected for Vp with real-time PCR.The tdh,trh,and orf8 genes were also tested.Results The results showed that the 43 genes of Vp were detected and the detection rate was 32.6%(43/132).The tdh genes were detected in 2 out of 43 isolates of Vp and the detection rate was 4.7%.The trh genes were detected in 2 out of 43 isolates of Vp and the detection rate was 4.7%.The orf8 genes were detected in 1 out of 43 isolates of Vp,and the detection rate was 2.3%.The orf8 genes and the tdh genes were detected in 1 out of 43 isolates of Vp and the detection rate was 2.3%.The trh genes and tdh genes were detected in 16 out of 43 isolates of Vp,and the detection rate was 37.2%.The tl genes were detected in 23 out of 80 isolates of Vp in oyster samples and the detection rate was 28.8%,including 10 samples containing virulence genes.Conclusion Heavy Vp contamination exists in seashells from coastal area of Dalian.

     

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