Abstract: Objective To establish silencer model in Beagle dogs and to evaluate physiological function of Beagle dogs with right recurrent laryngeal nerve amputation.Methods Twelve healthy Beagle dogs were randomly divided into a model and a control group;right recurrent laryngeal nerve amputation was performed in the dogs of the model group.Barking volume was measured for all the Beagle dogs at 3- and 6-month of the experiment and electrocardiogram,respiration function,and physiological and biochemical indicators of blood of the Beagle dogs were determined simultaneously.Results The barking volumes were 41.83±4.87 and 48.62±2.19 dB(A)for the control group and 33.04±2.34 and 35.20±1.98 dB(A)for the model group at 3- and 6-month,with significantly lower volumes in the Beagle dogs of model group compared to those of the control group(both P<0.05).No significant differences in electrocardiogram,respiration function,22 blood physiological indicators,and 8 blood biochemical indicators were observed between the control and model groups at 3- and 6-month of the experiment(P>0.05 for all).Conclusion Unilateral recurrent laryngeal nerve amputation can significantly reduce barking volume and has no significant impacts on electrocardiogram,respiration function,and hematologic indicators in Beagle dogs.